New paper in Langmuir

la-2016-02832w 0007We are pleased to announce a new research article, "Chaperonin-Inspired pH Protection by Mesoporous Silica SBA-15 on Myoglobin and Lysozyme," published by NICE group members Michele Lynch, Jichuan Liu, Michael Nigra, and Marc-Olivier Coppens in Langmuir, online from 26 August 2016.




While enzymes are valuable tools in many fields of biotechnology, they are fragile and must be protected against denaturing conditions such as unfavorable solution pH. Within living organisms, chaperonins help enzymes fold into their native shape and protect them from damage. Inspired by this natural solution, mesoporous silica SBA-15 with different pore diameters is synthesized as a support material for immobilizing and protecting enzymes.


In separate experiments, the model enzymes myoglobin and lysozyme are physically adsorbed to SBA-15 and exposed to a range of buffered pH conditions. The immobilized enzymes' biocatalytic activities are quantified and compared to the activities of nonimmobilized enzymes in the same solution conditions. It has been observed that myoglobin immobilized on SBA-15 is protected from acidic denaturation from pH 3.6 to 5.1, exhibiting relative activity of up to 350%. Immobilized lysozyme is protected from unfavorable conditions from pH 6.6 to 7.6, with relative activity of up to 200%.


These results indicate that the protective effects conferred to enzymes immobilized by physical adsorption to SBA-15 are driven by the enzymes' electrostatic attraction to the material's surface. The pore diameter of SBA-15 affects the quality of protection given to immobilized enzymes, but the contribution of this effect at different pH values remains unclear.

She will use ARCHER supercomputer for 6 months to study behavior of biomolecules confined in ordered amorphous silica through all-atom molecular dynamic simulations with explicit water and counter-ions.The simulations will provide a better understanding of the interplay between the adsorbed proteins and mesoporous materials which is necessary to improve drug delivery systems by improving stability and controlled release of the active drug at the site of interest.